Description | Anti-GUINEA PIG IgG (H&L) (DONKEY) Antibody Alkaline Phosphatase Conjugated (Min X Bv Ch Gt Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) |
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Host | Donkey |
Conjugate | Alkaline Phosphatase (Calf Intestine) |
Target Species | Guinea Pig |
Reactivity | Guinea Pig |
Clonality | Polyclonal |
Application
| WB, IHC, E |
Application Note | ELISA 1:2,000-1:10,000 Western Blot 1:500-1:2,500 Immunohistochemistry 1:200-1:1,000 |
Physical State | Liquid (sterile filtered) |
Host Isotype | IgG |
Target Isotype | IgG (H&L) |
Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
Immunogen | Guinea Pig IgG whole molecule |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.1% (w/v) Sodium Azide |
Shipping Condition | Wet Ice |
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Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Guinea Pig IgG and with light chains of most Guinea Pig immunoglobulins. |
Storage Condition | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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Cat# ASR3045