Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Methylation of chloroplast DNA of Chlamydomonas me-1 cells, visualized by anti-5-methylcytosine antibodies. Left: DAPI stained cells. Middle: Cells stained with anti-5MeC antibodies, followed by secondary anti-mouse IgM, FITC conjugated secondary antibodies, Right: Merged image. Chloroplast DNA is exclusively methylated in gamete cells. Described in Nishiyama et al. 2002.
Intense 5-methylcytosine staining at pericentromeric regions of mouse embryonic stem cells was seen in the mitotic chromosome and interphase nuclei of ESCs. Described in Sharif et al. 2007.
Background
Background:
DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA — for example, to the number 5 carbon of the cytosine pyrimidine ring — which involves reduction in gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells.
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