4-Hydroxynonenal monoclonal antibody, clone 12F7 (ATTO 488)
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- More Files
Immunocytochemistry
Immunocytochemical staining of HEK293 with 4-Hydroxynonenal monoclonal antibody, clone 12F7 (Cat # MAB19282). (A, B) DAPI (blue) nuclear stain, (C, D) Phalloidin Alex Fluor 633 F-Actin stain, (E, F) 4-Hydroxynonenal Antibody and (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.
- Specifications
Product Description
Mouse monoclonal antibody raised against synthetic 4-Hydroxynonenal (4-HNE).
Immunogen
Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
Host
Mouse
Reactivity
Human
Form
Liquid
Conjugation
ATTO 488
Purification
Protein G Purified
Isotype
IgG1
Recommend Usage
ELISA (1:1000)
Immunocytochemistry (1:50)
Immunofluorescence (1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot
Immunocytochemistry
Immunocytochemical staining of HEK293 with 4-Hydroxynonenal monoclonal antibody, clone 12F7 (Cat # MAB19282). (A, B) DAPI (blue) nuclear stain, (C, D) Phalloidin Alex Fluor 633 F-Actin stain, (E, F) 4-Hydroxynonenal Antibody and (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay