2×TransStart® GoldPfu PCR SuperMix (-dye)

Cat# AS401-02

Size : 5×1ml

Brand : TransGen Biotech

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TransStart® GoldPfu PCR SuperMix (-dye)

Catalog Number: AS401-01

Price:Please inquire first

Specification:
1 ml
5×1 ml
15×1 ml

Product Details

This product is a ready-to-use mixture of TransStart® GoldPfu DNA polymerase, dNTPs, and optimized reaction buffer showing high amplification efficiency, fast amplification, ultra high fidelity and high specificity. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and Nuclease-free Water. The amplified products with blunt ends can be directly cloned into pEASY®-Blunt vectors. The SuperMix without dye (-dye) cannot be directly loaded on agarose gel for electrophoresis.

Its PCR product is not suitable for polyacrylamide gel electrophoresis. 


  • Reduced PCR operation time.

  • Avoid contamination due to multi-step operation.

  • 54-fold fidelity as compared to EasyTaq® DNA Polymerase.

  • Amplification of genomic DNA fragment up to 15 kb.

  • Amplification of plasmid DNA fragment up to 20 kb.

Highlights

Fast amplification, ultra high fidelity, high specificity, high stability


Applications

  • Ultra High fidelity PCR

  • Site-directed mutagenesis

  • Blunt end cloning

  • Complex templates

  • GC/ AT rich templates

  • Long fragment PCR


Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)


Product Composition


ComponentAS401-01AS401-02AS401-03
TransStart® GoldPfu PCR SuperMix (-dye)        1 ml    5×1 ml  15×1 ml
Nuclease-free Water        1 ml        5 ml    3×5 ml


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