Nitric oxide (NO) is a crucial signaling molecule involved in various physiological and metabolic processes, including vasodilation, immune response, neurotransmission, and cellular respiration. As a short-lived free radical, NO plays a significant role in oxidative stress, inflammation, and metabolic homeostasis. Measuring NO levels is essential for understanding its impact on cardiovascular health, immune function, and metabolic disorders.
Principle of the Nitric Oxide Assay Kit
Since NO is highly reactive and has a short half-life, its levels are indirectly measured by detecting its stable metabolites, nitrite (NO₂⁻) and nitrate (NO₃⁻). The most common detection methods include:
- Colorimetric Assay: Nitrite reacts with reagents, producing a color change measurable by spectrophotometry.
- Fluorometric Detection: Offers higher sensitivity by using fluorescent probes that react with NO derivatives.
Applications in Metabolism and Disease Research
- Cardiovascular Health & Vasodilation: NO is a major regulator of blood vessel relaxation and circulation.
- Oxidative Stress & Inflammation: Plays a dual role as both an antioxidant and a pro-oxidant in metabolic disorders.
- Mitochondrial Function & Energy Metabolism: Modulates mitochondrial respiration and ATP production.
- Immune System Response: Acts as a defense molecule in macrophages against pathogens.
- Neurotransmission & Brain Function: Involved in synaptic plasticity and neuroprotection.
Advantages of Using a Nitric Oxide Assay Kit
- Highly Sensitive & Specific: Detects low NO levels with high accuracy.
- Multiple Sample Compatibility: Suitable for plasma, serum, tissue extracts, and cell culture supernatants.
- Reliable & Reproducible: Provides consistent and precise results.
- Fast & Easy-to-Use Protocol: Designed for efficiency in research settings.
