Reagents and instruments for immunology, cell biology and molecular biology.
 
Human cDNA clones

Human cDNA clones

Human cDNA clones represent recombinant DNA molecules containing complementary DNA (cDNA) sequences synthesized from human messenger RNA (mRNA), capturing protein-coding genes without introns for functional studies and expression.

Definition and Synthesis

cDNA clones derive from reverse transcription of poly(A)+ mRNA using reverse transcriptase, yielding first-strand cDNA extended by DNA polymerase for double-stranded molecules, then ligated into vectors such as plasmids or lambda phage. Human libraries from tissues (brain, liver) or cell lines provide full-length or partial clones, often with 5'/3' UTRs. Collections like hORFeome or CCSB-Broad encompass more than 30,000 verified human ORFs in Gateway-compatible entry vectors.

Characteristics

Clones feature correct reading frames (70% in high-quality libraries), His-tags for purification, sizes ranging from 0.5-10 kb matching predicted proteins (15-200 kDa), and verified inserts via Sanger or next-generation sequencing. Full-length clones prioritize complete ORFs, splicing variants capture isoforms, and solubility screening identifies E. coli-expressible proteins (~25% success rate).

Construction and Screening

Libraries array 105-106 clones on membranes for immunological detection of His-fusions, followed by small-scale expression and purification. For example, 2,746 out of 10,825 clones from the hEx1 library expressed soluble proteins ≥15 kDa. Transfer to lentiviral, baculoviral, or mammalian vectors (e.g., pcDNA) enables overexpression. Structural genomics selects clones for crystallization (e.g., 17 out of 163 yielded 3 structures).

Applications

Human cDNA clones facilitate protein production for X-ray crystallography and NMR, functional assays, high-throughput arrays, and gene therapy vectors. They define isoforms (e.g., N-CAM 120/140/180 kDa via splicing) and support transcriptomics studies. 

 


Frequently Asked Questions (FAQ)

Where can I find human products on the Biovalley website?

human products are available in this section and can be sorted by product families or sub-categories, such as Tagged. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.

What types of products are available in the human category?

The human category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Tagged sub-categories allow you to find the products that meet your needs more quickly.

How can I quickly find a human product?

To find a human product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Biovalley stock several product codes in the human category?

Yes, Biovalley distributes several product references in the human category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for human products?

Yes, you can request a quote for human products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a human product is available to order?

The availability of a human product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.

What should I do if I cannot find the human product I am looking for?

If the human product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all human products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.