The NeoPRO Western Substrate, developed by Neo Biotech, is a luminol-based enhanced chemiluminescent substrate that demonstrates exceptional sensitivity and compatibility for immunoblots using horseradish peroxidase (HRP) – conjugated secondary antibodies. This product enables the detection of antigens in the mid-femtogram to the high-femtogram range, thanks to its superior sensitivity and prolonged signal duration. Moreover, the NeoPRO Western Substrate's extended chemiluminescent signal duration allows for imaging using digital and film-based techniques without signal loss. For optimal signal intensity and duration, we recommend employing appropriate dilutions of primary and secondary antibodies.

Features

● No optimization required. Switching to the NeoPRO Western Substrate from other brands, such as Pierce and GE Healthcare, does not require optimization or protocol changes.

● High degree of sensitivity and enhanced chemiluminescence duration. NeoPRO Western Substrate enables an accurate mid-femtogram to high-femtogram detection of protein on the same immunoblot after a single exposure.

● Optimized for use with PVDF and nitrocellulose membranes.

● Compatible with Western Blotting Markers.

● Optimized for a film- and CCD-based imaging

Researchers can choose between the NeoPro FEMTO Western Substrate and the NeoPro Pico Ultra Western ECL Substrate based on their specific sensitivity needs. Both substrates provide advanced chemiluminescent capabilities and signal longevity, ensuring optimal results for a wide range of immunoblotting applications.