ROS Detection Assay Kit (DCFDA / H2DCFDA)
Cat# CA093
Size : 5×96assays
Brand : Canvax Biotech
Contact local distributor :
Phone : +1 850 650 7790
IPTG
>99.0% Purity confirmed by HPLC & Dioxane free
IPTG (Isopropyl B-D-thiogalactopyranoside), Dioxane-Free is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. Unlike allolactose, IPTG is not a substrate for b-galactosidase, as the carbon-sulfur bond cannot be cleaved by the enzyme, so its concentration remains constant. The compound is widely used with X-Gal plates to screen for blue/white selection of recombinant bacteria.
Detailed information:
Advantages & Features
- Purity: >99.0% confirmed by HPLC.
- Dioxane free: does not disrupt normal cell function.
- High Quality.
- Powder format.
Specifications
- CAS Number: 367-93-1
- Chemical Formula: C9H18O5S
- Molecular Weight: 238.30
- Purity (HPLC)(on dry basis): >99.0%
- Melting point: 110 – 114 °C
- Identity (IR): conforms to structure
- Solubility: soluble in water and methanol
- Heavy metals (Pb): <5ppm
- 1,4-Dioxane: Not detected
- pH (5% in water): 5.0 – 7.0
- Water content (Karl Fischer): <1.0%
Includes
– 25 g IPTG
Download documentation
Datasheet MSDSApplications
- Blue/white screening.
- Expression of genes under lac promoter control.
Tables & Figures
Quality Control
- Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Advice
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: 4 ºC .
Citations
- Pacheco-Garcia, J. L., Anoz-Carbonell, E., Loginov, D. S., Vankova, P., Salido, E., Man, P., … & Pey, A. L. (2022). Different phenotypic outcome due to site-specific phosphorylation in the cancer-associated NQO1 enzyme studied by phosphomimetic mutations. Archives of Biochemistry and Biophysics, 109392.
- Pacheco-García, J. L., Loginov, D. S., Naganathan, A. N., Vankova, P., Cano-Muñoz, M., Man, P., & Pey, A. L. (2022). Perturbation analysis in the N-terminal domain of hPGK1: a biophysical, structural and statistical mechanical study.
- Pacheco-Garcia, J. L., Loginov, D. S., Anoz-Carbonell, E., Vankova, P., Palomino-Morales, R., Salido, E., … & Pey, A. L. (2022). Allosteric Communication in the Multifunctional and Redox NQO1 Protein Studied by Cavity-Making Mutations. Antioxidants, 11(6), 1110.
- Pacheco-García, J. L., Cano-Muñoz, M., Sánchez-Ramos, I., Salido, E., & Pey, A. L. (2020). Naturally-Occurring Rare Mutations Cause Mild to Catastrophic Effects in the Multifunctional and Cancer-Associated NQO1 Protein. Journal of Personalized Medicine, 10(4), 207.
- Blanco, S., Peralta, S., Morales, M. E., Martínez-Lara, E., Pedrajas, J. R., Castán, H., … & Ruiz, M. A. (2020). Hyaluronate Nanoparticles as a Delivery System to Carry Neuroglobin to the Brain after Stroke. Pharmaceutics, 12(1), 40.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.