Raffinose Pentahydrate, Low Glucose (D-(+)-Raffinose, Melitose, Melitriose)

Cat# R1030-1Kg

Size : 1Kg

Brand : US Biological

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R1030 Rabbit Anti-Raffinose Pentahydrate, Low Glucose (D-(+)-Raffinose, Melitose, Melitriose)

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Shipping Temp
RT
Storage Temp
RT

Used as an alternate carbon source for wild-type yeast. Raffinose is a trisaccharide composed of galactose, glucose, and fructose. It can be found in beans, cabbage, brussels sprouts, broccoli, asparagus, other vegetables, and whole grains. Raffinose can be hydrolyzed to D-galactose and sucrose by the enzyme α-galactosidase (α-GAL), an enzyme not found in the human digestive tract. α-GAL also hydrolyzes other α-galactosides such as stachyose, verbascose, and galactinol, if present. The enzyme does not cleave β-linked galactose, as in lactose.||The raffinose family of oligosaccharides (RFOs) are alpha-galactosyl derivatives of sucrose, and the most common are the trisaccharide raffinose, the tetrasaccharide stachyose, and the pentasaccharide verbascose. RFOs are almost ubiquitous in the plant kingdom, being found in a large variety of seeds from many different families, and they rank second only to sucrose in abundance as soluble carbohydrates.||Synonyms:|D-(+)-Raffinose; Melitose, Melitriose; O-a-D-Galactopyranosyl-(1->6)-a-D-glucopyranosyl-ß-D-fructofuranoside||CAS No:|17629-30-0||Molecular Formula:|C18H32O16•5H2O||Molecular Weight:|594.51||Purity:|≥98%||Appearance:|White, crystalline powder||Solubility (10%):|Colorless, clear, complete after autoclaving||Glucose: |≤0.02%||Source:|Gossypium hirsutum L.||Identification (FTIR):|Complies||Loss on Drying: |13-17%||Residue on Ignition: |0.1%||Specific Rotation:|+103° to +108°||Melting Point:|78-82°C||Heavy Metals:|≤0.001%||Lead (Pb):|≤0.0002%||Arsenic (As):|≤0.0002%|||Storage:|RT|||Meets or Exceeds USP/NF Specifications

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
References
References:|Storey B., Noiles, E., Thompson, K. (1998). "Comparison of Glycerol, Other Polyols, Trehalose, and Raffinose to Provide a Defined Cryoprotectant Medium for Mouse Sperm Cryopreservation". Cryobiology. 37 (1): 46–58. doi:10.1006/cryo.1998.2097. PMID 9698429.|2. ”P-Body Components Are Required for Ty1 Retrotransposition during Assembly of Retrotransposition-Competent Virus-Like Particles“, Mary Ann Checkley, Kunio Nagashima, Stephen J. Lockett, Katherine M. Nyswaner and David J. Garfinkel, Mol. Cell. Biol. January vol. 30 no. 2 382-398 (2010)|3. ”Saccharomyces cerevisiae JEN1 Promoter Activity Is Inversely Related to Concentration of Repressing Sugar“, Prima Chambers, Aminatu Issaka, and Sean P. Palecek, Appl Environ Microbiol. 2004 Jan; 70(1): 8–17. doi:  10.1128/AEM.70.1.8-17.2004, PMCID: PMC321234|4. ”Quantitative Analysis of Membrane Trafficking in Regulation of Cdc42 Polarity|Leah J. Watson, Guendalina Rossi, and Patrick Brennwald“, Traffic. 2014 Dec; 15(12):1330–1343.|Published online 2014 Oct 8. doi:  10.1111/tra.12211, PMCID: PMC4260267, NIHMSID: NIHMS646090|5. Ignea C, et al. Overcoming the plasticity of plant specialized metabolism for selective diterpene production in yeast. Sci Rep. 2017; 7: 8855. doi: 10.1038/s41598-017-09592-5|6. 5. Owes S., et al. Monitoring Recombination During Meiosis in Budding Yeast. Methods in Enzymology. Available online 1 Feb 2018. https://doi.org/10.1016/bs.mie.2017.12.005.

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