Mouse IgG1 Isotype Control
Cat# AM12069LE-N
Size : 100ug
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Mouse IgG1 Isotype Control
| Product Data | |
| Application | C, FC, IP, P, WB |
|---|---|
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Specificity | This mouse IgG1 kappa antibody has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed tissues. |
| Buffer | Azide free phosphate buffered saline (PBS), approx. pH 7.4; purified by protein A affinity chromatography |
| Concentration | 1 mg/ml |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| presentation_state | Low Endotoxin |
| Storage | Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
| Background | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |