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> HSP60 (Heat Shock Protein 60)

HSP60 (Heat Shock Protein 60)

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Cat# NB-26-02011

Size : 100ul

Brand : Neo Biotech

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Phone : +1 850 650 7790

Product Details
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:5,000; Immunohistochemistry: 1:5,000; Western Blot: 1:5,000-10,000
Immunogen: Recombinant full length human HSP60 expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Primate, Bovine, Dog, Porcine, Equine
Entrez:
UniProt:
Immunofluorescence

Confocal immunofluorescent analysis of HeLa cells stained with rabbit pAb to HSP60, RA22149, dilution 1:1,000, in red, and costained with chicken pAb to vimentin in green. The blue is DAPI staining of nuclear DNA. The HSP60 antibody gives strong and specific staining of mitochondria while the vimentin antibody reveals cytoplasmic intermediate filaments.

Immunofluorescence

Confocal immunofluorescent analysis of HeLa cells stained with rabbit pAb to HSP60, RA22149, dilution 1:1,000, in red, and costained with chicken pAb to vimentin in green. The blue is DAPI staining of nuclear DNA. The HSP60 antibody gives strong and specific staining of mitochondria while the vimentin antibody reveals cytoplasmic intermediate filaments.

Western Blot

Western blot analysis of different tissue or cell lysates using rabbit pAb to HSP-60, RA22149, dilution 1:5,000 in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong 60kDa band present in all preparations corresponds to HSP60 protein.

Western Blot

Western blot analysis of different tissue or cell lysates using rabbit pAb to HSP-60, RA22149, dilution 1:5,000 in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong 60kDa band present in all preparations corresponds to HSP60 protein.