Reagents and instruments for immunology, cell biology and molecular biology.
 
> Goat Anti-Rabbit IgM-AP

Goat Anti-Rabbit IgM-AP

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Cat# 4020-04

Size : 1.0mL

Brand : Southern Biotech

Contact local distributor :


Phone : +1 850 650 7790

Product Specifications

More Information
Isotype Goat IgG
Isotype Control 0109-04
Specificity Reacts with the heavy chain of rabbit IgM
Source Pooled antisera from goats hyperimmunized with rabbit IgM
Cross Adsorption Rabbit IgG; may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rabbit IgM covalently linked to agarose
Conjugate AP (Alkaline Phosphatase)
Buffer Formulation 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-5
FLISA

Other referenced applications for relevant formats include -
Flow Cytometry 6-13
ELISPOT 3,13
Immunohistochemistry-Frozen Sections 14,15
Immunohistochemistry-Paraffin Sections 16
Western Blot 17-20
Separation 10,21
RRID Number AB_2795927

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

Other Conjugates and Formulations

UNLB FITC TRITC HRP BIOT PE AF647 AF555

Citations

  1. 1. Holdsworth RJ, McKenzie H, Parratt D, Irving AD, Cuschieri A. The role of the spleen in the immune response following naturally acquired exposure to encapsulated bacteria. Int J Exp Pathol. 1990;71:835-43. (ELISA)
  2. 2. Taylor-Robinson AW, Phillips RS. B cells are required for the switch from Th1- to Th2-regulated immune responses to Plasmodium chabaudi chabaudi infection. Infect Immun. 1994;62:2490-98. (ELISA)
  3. 3. Yamashita T, Freigang S, Eberle C, Pattison J, Gupta S, Napoli C, et al. Maternal immunization programs postnatal immune responses and reduces atherosclerosis in offspring. Circ Res. 2006;99:e51-64. (ELISA, ELISPOT)
  4. 4. Quan JH, Hassan HA, Cha G, Shin D, Lee Y. Antigenemia and specific IgM and IgG antibody responses in rabbits infected with Toxoplasma gondii. Korean J Parasitol. 2009;47:409-12. (ELISA)
  5. 5. Isik G, Sliepen K, van Montfort T, Sanders RW. Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL. PLoS One. 2014;9(9):e107683. (ELISA)
  6. 6. Pospisil R, Young-Cooper GO, Mage RG. Preferential expansion and survival of B lymphocytes based on VH framework 1 and framework 3 expression: "positive" selection in appendix of normal and VH-mutant rabbits. Proc Natl Acad Sci USA. 1995;92:6961-5. (FC)
  7. 7. Sehgal D, Mage RG, Schiaffella E. VH mutant rabbits lacking the VH1a2 gene develop a2+ B cells in the appendix by gene conversion-like alteration of a rearranged VH4 gene. J Immunol. 1998;160:1246-55. (FC)
  8. 8. Yang G, Obiakor H, Sinha RK, Newman BA, Hood BL, Conrads TP, et al. Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix. Proc Natl Acad Sci USA. 2005;102:17083-8. (FC)
  9. 9. Pospisil R, Kabat J, Mage RG. Characterization of rabbit CD5 isoforms. Mol Immunol. 2009;46:2456-64. (FC)
  10. 10. Yang J, Pospisil R, Mage RG. Expression and localization of rabbit B-cell activating factor (BAFF) and its specific receptor BR3 in cells and tissues of the rabbit immune system. Dev Comp Immunol. 2009;33:697-708. (FC, Sep)
  11. 11. Yang J, Pospisil R, Ray S, Milton J, Mage RG. Investigations of a rabbit (Oryctolagus cuniculus) model of systemic lupus erythematosus (SLE), BAFF and its receptors. PLoS One. 2009;4(12):e8494. (FC)
  12. 12. Mage RG, Rai G. A rabbit model of systemic lupus erythematosus, useful for studies of neuropsychiatric SLE. Systemic Lupus Erythematosus, Almoallim H ed. 2012. (FC)
  13. 13. Baranyi M, Cervenak J, Bender B, Kacskovics I. Transgenic rabbits that overexpress the neonatal Fc receptor (FcRn) generate higher quantities and improved qualities of anti-thymocyte globulin (ATG). PLoS One. 2013;8(10):e76839. (FC, ELISPOT)
  14. 14. Schiaffella E, Sehgal D, Anderson AO, Mage RG. Gene conversion and hypermutation during diversification of VH sequences in developing splenic germinal centers of immunized rabbits. J Immunol. 1999;162:3984-95. (IHC-FS)
  15. 15. Sehgal D, Obiakor H, Mage RG. Distinct clonal Ig diversification patterns in young appendix compared to antigen-specific splenic clones. J Immunol. 2002;168:5424-33. (IHC-FS)
  16. 16. Rosner A, Moiseeva E, Rabinowitz C, Rinkevich B. Germ lineage properties in the urochordate Botryllus schlosseri – From markers to temporal niches. Dev Biol. 2013;384:356-74. (IHC-PS)
  17. 17. Gibson JR, Chart H, Owen RJ. Intra-strain variation in expression of lipopolysaccharide by Helicobacter pylori. Lett Appl Microbiol. 1998;26:399-403. (WB)
  18. 18. Chart H, Cheasty T, Willshaw GA. Production of serum antibodies that recognise epitopes located on the R3 region of Escherichia coli core lipopolysaccharides by patients infected with strains of enterohaemorrhagic E. coli. J Med Microbiol. 2002;51:1050-4. (WB)
  19. 19. Chart H, Cheasty T, de Pinna E, Siovanes L, Wain J, Alam D, et al. Serodiagnosis of Salmonella enterica serovar Typhi and S. enterica serovars Paratyphi A, B and C human infections. J Med Microbiol. 2007;56:1161-6. (WB)
  20. 20. Furuya K, Omura M, Kudo S, Sugiura W, Azuma H. Recognition profiles of microsporidian Encephalitozoon cuniculi polar tube protein 1 with human immunoglobulin M antibodies. Parasite Immunol. 2008;30:13-21. (WB)
  21. 21. Pospisil R, Fitts MG, Mage RG. CD5 is a potential selecting ligand for B cell surface immunoglobulin framework region sequences. J Exp Med. 1996;184:1279-84. (Sep)