Application 
| WB, E |
|---|---|
| Reactivity | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure recombinant Rat GRO/KC. Anti-Rat GRO/KC specific antibody was purified by affinity chromatography and then biotinylated. |
|---|---|
| WesternBlot | To detect Rat GRO/KC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat GRO/KC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich | To detect Rat GRO/KC by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Rat GRO/KC (62-059P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat GRO/KC. |
| Direct | To detect Rat GRO/KC by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat GRO/KC. |
| Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage | -20°C |
| Precautions | Biotinylated Anti-Rat GRO/KC Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |