Actin

Cat# NB-26-00460-100UG

Size : 100ug

Brand : Neo Biotech

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Product Details
Applications: WB, IF
Type: Mouse IgG
Immunogen: Actin preparation derived from bovine brain.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez:
UniProt:

HeLa cells stained with Actin (red) and also with our chicken polyclonal antibody to Vimentin, (green) (CH22108). The actin antibody stains the submembranous actin rich cytoskeleton and also stress fibers, bundles of actin associated with adhesion sites. The vimentin antibody stains a quite different cytoskeletal network, the intermediate filaments. The blue stain reveals DNA in the nuclei of these cells. Protocol on Datasheet.

HeLa cells stained with Actin (red) and also with our chicken polyclonal antibody to Vimentin, (green) (CH22108). The actin antibody stains the submembranous actin rich cytoskeleton and also stress fibers, bundles of actin associated with adhesion sites. The vimentin antibody stains a quite different cytoskeletal network, the intermediate filaments. The blue stain reveals DNA in the nuclei of these cells. Protocol on Datasheet.

Western blot of crude extract of the human carcinoma HeLa cell line. Lane 15 was probed with Actin, our monoclonal antibody to all six actin isotypes, giving a strong clean band at an SDS-PAGE molecular weight of 42kDa. lane 16 was probed with our antibody to 14-3-3η (14-3-3 eta) (MO22126), which has an SDS-PAGE molecular weight of 28kDa. Lanes 17 was probed with our monoclonal antibody to vimentin (CH22108). Protocol on Datasheet.

Western blot of crude extract of the human carcinoma HeLa cell line. Lane 15 was probed with Actin, our monoclonal antibody to all six actin isotypes, giving a strong clean band at an SDS-PAGE molecular weight of 42kDa. lane 16 was probed with our antibody to 14-3-3η (14-3-3 eta) (MO22126), which has an SDS-PAGE molecular weight of 28kDa. Lanes 17 was probed with our monoclonal antibody to vimentin (CH22108). Protocol on Datasheet.