Reagents and instruments for immunology, cell biology and molecular biology.
 
µ-Plate 384 Well Black Glass Bottom

µ-Plate 384 Well Black Glass Bottom

A black 384-well plate with square wells and a #1.5H glass coverslip bottom for high-throughput screening using TIRF and super resolution microscopy

  • Compatible with robotics, thanks to a standard ANSI/SLAS (SBS) format
  • Brilliant cell imaging due to the low thickness variability of the glass coverslip #1.5H and the excellent flatness of the inner well and the whole plate
  • Low well-to-well crosstalk in fluorescence microscopy

Applications

  • Culture, high throughput screening (HTS) and high resolution microscopy of cells
  • Wide field fluorescence imaging and confocal microscopy of living and fixed cells
  • Compound testing (toxicology)
  • Large-scale transfection experiments
  • Live cell imaging, also suitable for extended periods
  • Total internal reflection fluorescence (TIRF) and single molecule applications
  • Super-resolution microscopy (STED, SIM, (F) PALM, (d) STORM) and fluorescence correlation spectroscopy (FCS)
  • Immunofluorescence staining

Technical specifications

  • Standard format, compatible with robotics and plate readers thanks to a standard ANSI/SLAS (SBS) format
    (85,5 x 127,5 mm)
  • 384 quadratic wells with standard numbering
    (letters A-P and numbers 1-24)
  • Suitable for fluorescence scanners, plate readers, robotics and laboratory automation
  • Schott D 263 M glass bottom with a thickness of 170 µm +/- 5 µm
  • May require a coating to promote cell attachment
  • No autofluorescence
  • Excellent flatness of the inner well and flatness of the whole plate
  • Compatible with staining and fixing solvents
  • Non-cytotoxic and biocompatible
  • Compatible with immersion oil
  • Sterile with single packaging

 

 

 

The principle of the µ-Plate 384 Well Black Glass Bottom

The bottom of the slide

The µ-Plate 384 Well Black Glass Bottom has optimal flatness properties, which are crucial for high-resolution microscopy in screening applications. The glass coverslip offers high precision and facilitates imaging from well to well across the plate with less focus variation due to the rigid material with high mechanical stability.

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