µ-Slide 15 Well 3D
A chambered coverslip with 15 wells for 3D cell culture, tube formation tests and immunofluorescence staining
- Bright visualization of cells without gel meniscus formation and with all cells in one focal plane
- Ideal for 3D cell culture with a wide range of gels (for example, Matrigel ® , collagen and agarose)
- Cost-effective experiments, requiring only 10 µl of gel per well
Applications
- High rate tube formation tests
- 3D cell culture
- Immunofluorescence staining
- Germination tests
- Live cell imaging of attached cells
Technical characteristics
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Focus on each cell: the principle of the µ-Slide 15 Well 3D
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The μ-Slide 15 Well 3D has a specialized geometry for easy, convenient and reproducible testing of tube formation. It is also ideal for germination tests, immunofluorescence staining and general and 3D cell culture. Once the gel has been pipetted into the inner well and has had time to solidify, the cells can be seeded on it for tube on top for analysis of the tube formation . Thanks to the well-in-a-well technology, the amount of gel required is reduced to only 10 μl per well, or 10% of the amount used in conventional multi-well plates. In addition, no gel meniscus is formed. This ensures the formation of a uniformly thick gel matrix on which all cells are in an optical plane, creating reproducible cell culture conditions. The µ-Slide 15 Well 3D can be used with used with all common hydrogel matrices. Such as Matrigel ® , collagen gels, agarose dels and hyaluronic acid gel. With its 15 wells, the µ-Slide 15 Well 3D is designed for low flow rate assays. For large-scale applications ibidi provides the μ-Plate 96 Well 3D. |
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