Venor®GeM OneStep is a kit for direct detection of mycoplasma contaminations in cell cultures, cell culture media, and other biological matrices by conventional PCR. Designed to require minimal pipetting, the kit includes all reagents required for PCR: primers, nucleotides, polymerase and the internal amplification control, which are provided in a ready-to-use lyophilized reaction mix. Upon rehydration with the included buffer, the mix is ready to be dispensed into the reaction tubes according to the sample number. Only samples or controls need to be added prior to PCR.

Type of PCR

For conventional, endpoint PCR with gel electrophoresis

Recommended Use

Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Kit Components

Lyophilized Mix: Primer sets / Nucleotides / Internal Control DNA and polymerase at optimized concentrations in aliquots of 25 reactions each
Rehydration Buffer
Lyophilized Positive Control DNA
PCR Grade Water

Package Sizes

• Cat. No. 11-8025 25 Reactions
• Cat. No. 11-8050 50 Reactions
• Cat. No. 11-8100 100 Reactions
• Cat. No. 11-8250 250 Reactions

Result Evaluation

Gel electrophoresis at endpoint of PCR

Required Consumables

Agarose gels and DNA stains
PCR reaction tubes and pipette filter tips

Required Lab Devices

PCR cycler for conventional PCR
Gel electrophoresis and documentation system
Pipetting equipment
Microcentrifuge

Shelf Life and Storage

Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.

EP 2.6.7 compliance

No

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