TransStart® Taq DNA Polymerase (with 2.5 mM dNTPs)
Référence AP141-11
Conditionnement : 250units
Marque : TransGen Biotech
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
ManualTransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins. At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
• TransStart® Taq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Reduced nonspecific amplification and primer dimer formation.
• Different from Taq antibody, no risk of contamination from mammalian DNA.
• Different from chemical modification, long denaturing step is not needed.
• Amplification of genomic DNA fragment up to 15 kb.
Applications
• Complex templates
• GC/AT-rich templates
• Multiplex PCR
• High yield PCR
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AP141-01/11 | AP141-02/12 | AP141-03/13 |
| TransStart® Taq DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 10×TransStart® Taq Buffer | 1.2 ml | 1.2 ml×2 | 1.2 ml×12 |
| 2.5 mM dNTPs | -/800 μl×1 | -/800 μl×2 | -/1.2 ml×8 |
| 10×GC Enhancer | 200 μl×1 | 400 μl×1 | 1 ml×1 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml×1 | 1 ml×2 |
Li S , Zhou L , Yao Y , et al. A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays[J]. Chemical Communications, 2016, 53.
Yu C Y , Yin B C , Ye B C . A universal real-time PCR assay for rapid quantification of microRNAs via the enhancement of base-stacking hybridization[J]. Chemical Communications, 2013, 49(74):8247.
Bai C , Liu X , Fisher M C , et al. Global and endemic Asian lineages of the emerging pathogenic fungus Batrachochytrium dendrobatidis widely infect amphibians in China[J]. Diversity & Distributions, 2012, 18(3):307-318.
Lin X , Li N , Kudo H , et al. Genes Sufficient for Synthesizing Peptidoglycan are Retained in Gymnosperm Genomes, and MurE from Larix gmelinii can Rescue the Albino Phenotype of Arabidopsis MurE Mutation[J]. Plant and Cell Physiology, 2017, 58(3):587-597.
Song Y , Zhao G , Zhang X , et al. The crosstalk between Target of Rapamycin (TOR) and Jasmonic Acid (JA) signaling existing in Arabidopsis and cotton[J]. Scientific Reports, 2017, 7:45830.