TransScript^® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for the detection and quantification of miRNA from total RNA, small RNA or other miRNA containing samples. TransScript^® miRNA RT Enzyme Mix (Poly(A) polymerase and reverse transcriptase are included) and 2×TS miRNA Reaction Mix are supplied to efficiently add Poly(A) tails and synthesize first-strand cDNA. PerfectStart^TM Green qPCR SuperMix is used for miRNA quantification.

Highlights

• Optimal ratio of Poly(A) polymerase to reverse transcriptase and optimized reaction buffer, so as to ensure high transcription efficiency of miRNA.

• One-step completion of Poly(A) tail addition and first-strand cDNA synthesis in a single tube.

• High amplification efficiency, specificity and sensitivity ensured by PerfectStart^TM Green qPCR SuperMix, leading to accurate data.

• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).

Applications
Multiple copy and low copy gene detection.

Storage
at -20°C  in dark for two years

Shipping
Dry ice (-70 ℃)