- More Files
- Specifications
Product Description
Mouse monoclonal antibody raised against native TRA-1-60.
Immunogen
Native purified from human embryonal carcinoma cell line 2102Ep.
Host
Mouse
Reactivity
Human
Specificity
TRA-1-60 recognizes antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope.
Form
Liquid
Isotype
IgM
Recommend Usage
Flow Cytometry (1:50-1:200)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Cell lysate)
Western blot analysis of TRA-1-60 expression using TRA-1-60 monoclonal antibody, clone TRA-1-60 (Cat #MAB2391) in NTERA-2 cl.D1 whole cell lysate.Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
- Publication Reference
- Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells.
Matin MM, Walsh JR, Gokhale PJ, Draper JS, Bahrami AR, Morton I, Moore HD, Andrews PW.
Stem Cells 2004 Apr; 22(5):659.
Application:IF, Human, 2102Ep, H14 cells.
- The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan.
Badcock G, Pigott C, Goepel J, Andrews PW.
Cancer Research 1999 Sep; 59(18):4715.
Application:WB-Ce, Human, 2102Ep cells.
- Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells.