Superoxide Dismutase (SOD) BioAssay™ Kit (WST-1 Method)

Superoxide dismutase plays a vital role in body balancing status of oxidation and antioxidation. This enzyme can remove superoxide anion free radical, protect cells from damage. The activity of SOD was measured by WST-1 method in this kit. Water-soluble tetrazolium, the sodium salt of 4-[3-(4iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1), was used as a detector of superoxide radical generated by xanthine oxidase and hypoxanthine. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.||Sample Type:|Serum, plasma, cerebrospinal fluid, pleural effusion, ascites, renal dialysis fluid, urine, semen, red blood cells, white blood cells, platelets, myocardial cells, tumor cells and a variety of plant and animal tissues and cells, subcellular level (mitochondria and microsome).||Intended Use:|This kit adopts the xanthine oxidase (hydroxylamine method) to measure SOD activity. The activity of SOD in Serum, plasma, cerebrospinal fluid, pleural effusion, ascites, renal dialysis fluid, urine, semen, red blood cells, white blood cells, platelets, myocardial cells, tumor cells and a variety of plant and animal tissues and cells, subcellular level (mitochondria and microsome) can be tested by this kit. And the activity of SOD in microorganisms, medicine, food, beverage, cosmetics and other samples can be tested by this kit too.||Sensitivity: |0.5U/mL.||Precision: |Intra-Assay CV: <5.50%|Inter-Assay CV: <3.32%||Kit Components (see Protocol for final kit quantities):|Reagent 1, 15mL × 2vial:|Buffer Solution||Reagent 2, 0.15mL × 1vial:|Substrate Solution||Reagent 3, 0.3mL ×1vial:|Enzyme Solution||Reagent 4, 4mL × 1vial:|Enzyme Diluent||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.