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Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Sucrose Fluorometric Assay Kit

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Référence E-BC-F042-96T

Conditionnement : 96T

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Marque : Elabscience

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Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Detection Principle	Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Sample Type	Plant tissue
Detection Method	Fluorometric method
Detection Instrument	Fluorescence microplate reader (Ex/Em=535 nm/590 nm)
Research Area	Glycolysis And Lipid Metabolism , Plant Physiology Series
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.15 μmol/L
Detection Range	0.15-15 μmol/L
Precision	inter-assay CV: 6.5% \| intra-assay CV: 2.3%
Assay Time	1 h 10 min

Cat.No.	Product Name	Sizes
E-BC-K161-S	Sucrose Colorimetric Assay Kit	100Assays , 50Assays
E-BC-K751-M	Sucrase Activity Assay Kit	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Corn tissue homogenate	1500
10% Potato tissue homogenate	300-500
10% Tomato tissue homogenate	200-300
10% Macrophanerophytes leaf tissue homogenate	200-400
10% Carrot tissue homogenate	1500
10% Onion tissue homogenate	500-1000
10% Green pepper tissue homogenate	500-1000
10% Bush leaves tissue homogenate	20-50

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.