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> Sucrose Colorimetric Assay Kit

Sucrose Colorimetric Assay Kit

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Référence E-BC-K161-S-50

Conditionnement : 50Assays

Marque : Elabscience

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Téléphone : +1 850 650 7790

Detection Principle Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.
Sample Type Plant tissue
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (290 nm)
Research Area Glycolysis And Lipid Metabolism
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.32 μmol/mL
Detection Range 0.32-70 μmol/mL
Precision inter-assay CV: 8.5% | intra-assay CV: 3.4%
Assay Time 40 min
Cat.No. Product Name Sizes
E-BC-K018-S D-Xylose Colorimetric Assay Kit 100Assays , 50Assays
E-BC-K234-S Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method) 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Green pepper tissue homogenization 1
10% Epipremnum aureum tissue homogenization 1
10% Cucumber tissue homogenization 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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