RC212285 | SMCR8 (NM_144775) Human Tagged ORF Clone Biovalley

SMCR8 (NM_144775) Human Tagged ORF Clone

SKU

RC212285

SMCR8 (Myc-DDK-tagged)-Human Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8)

Specifications
Product Data
Target Symbol	SMCR8
Synonyms	DENND8A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence >RC212285 representing NM_144775 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAGCGCCCCTGACGTAGTGGCCTTCACCAAAGAGGAAGAGTATGAAGAAGAGCCTTACAATGAGC CGGCCCTGCCTGAGGAGTACTCGGTGCCGCTCTTCCCCTTCGCCAGTCAGGGTGCTAACCCCTGGTCAAA ACTGTCCGGGGCCAAGTTTTCGAGGGACTTCATTCTTATTTCCGAGTTCTCTGAGCAGGTGGGACCCCAA CCCTTACTGACCATCCCCAATGACACCAAAGTTTTTGGCACTTTTGATCTCAATTACTTCTCCCTGCGTA TCATGTCTGTGGATTACCAGGCTTCCTTCGTGGGCCATCCTCCTGGATCTGCCTACCCCAAGCTGAACTT CGTGGAGGACTCCAAGGTGGTGCTGGGAGATTCTAAGGAGGGCGCCTTTGCATACGTGCACCACCTTACC CTATACGACCTGGAGGCCCGTGGCTTCGTGAGGCCGTTTTGCATGGCTTATATCTCTGCAGACCAGCATA AAATCATGCAGCAGTTCCAGGAGCTTTCAGCCGAATTTTCCAGAGCTTCTGAGTGCTTGAAGACTGGCAA CAGGAAGGCATTTGCTGGGGAACTTGAAAAAAAGCTGAAAGACTTGGATTACACCAGGACAGTGCTACAC ACAGAAACGGAGATCCAGAAGAAAGCCAACGACAAAGGCTTTTACTCATCTCAGGCAATTGAGAAAGCCA ATGAACTGGCCAGTGTGGAGAAGTCCATCATTGAACATCAAGACCTGCTGAAGCAGATCCGCTCATACCC TCATCGGAAGTTGAAGGGGCATGATTTGTGTCCTGGTGAGATGGAGCACATCCAGGATCAGGCCAGCCAG GCATCCACTACCTCTAACCCTGATGAGTCTGCCGACACAGACCTTTACACCTGCAGACCAGCCTACACCC CAAAACTTATCAAAGCAAAGTCCACCAAGTGTTTTGACAAGAAGTTGAAGACCTTGGAGGAGCTCTGTGA CACTGAATATTTCACCCAGACCCTGGCTCAGCTCAGCCACATTGAACACATGTTCAGAGGAGACCTGTGT TACCTCCTGACCAGTCAGATTGATAGAGCACTTCTAAAACAACAGCATATAACAAACTTTCTCTTTGAAG ACTTTGTGGAGGTCGATGACAGGATGGTGGAGAAACAAGAAAGCATACCCTCTAAGCCCAGTCAAGACAG GCCGCCTTCCAGTTCTCTAGAAGAATGCCCAATTCCTAAAGTGTTAATTAGTGTTGGTTCTTACAAGTCC AGTGTGGAGTCTGTGTTGATCAAGATGGAGCAGGAACTGGGAGATGAGGAGTACAAGGAAGTGGAAGTGA CTGAGTTGAGCAGTTTCGACCCCCAGGAAAACTTGGACTACCTGGATATGGATATGAAAGGGAGTATCAG CAGTGGTGAAAGTATTGAAGTTTTGGGCACGGAGAAATCCACCTCCGTGCTTTCTAAATCTGACAGCCAG GCAAGCCTCACAGTACCATTGAGCCCCCAGGTGGTCCGGAGCAAAGCAGTCAGCCACAGGACCATCAGCG AGGACAGTATTGAAGTCCTCAGTACCTGCCCCTCTGAGGCCCTCATCCCTGATGACTTTAAGGCCAGCTA CCCAAGTGCCATTAATGAAGAAGAATCATATCCAGATGGCAATGAAGGAGCCATCCGCTTCCAGGCAAGC ATCAGTCCTCCAGAACTGGGTGAGACAGAGGAAGGCAGCATAGAAAACACCCCATCACAAATAGACTCCT CCTGCTGTATTGGGAAGGAGAGCGATGGTCAGTTGGTGCTGCCCTCCACTCCAGCCCACACACACTCTGA CGAGGATGGGGTGGTGAGCAGCCCCCCACAGCGCCACAGGCAGAAGGACCAGGGGTTCCGTGTAGACTTT TCAGTGGAAAATGCCAACCCTTCTTCCCGAGACAACAGTTGTGAAGGGTTTCCCGCTTATGAGCTGGACC CGAGCCACCTGCTGGCTAGCCGGGACATCAGTAAGACCAGCCTGGACAACTACTCAGACACCACCAGCTA CGTGAGCAGTGTAGCGTCCACCAGCTCAGACAGGATCCCCTCTGCTTATCCTGCTGGCCTGTCTTCCGAT AGGCATAAAAAGAGGGCTGGCCAGAACGCCTTAAAATTCATCCGCCAGTACCCCTTTGCCCACCCAGCCA TCTACTCCCTGCTCAGTGGGAGGACACTTGTGGTCCTGGGGGAAGATGAGGCCATAGTCAGGAAACTCGT GACTGCACTGGCTATCTTTGTCCCCAGCTATGGCTGCTACGCTAAGCCCGTGAAACATTGGGCCTCCTCC CCTTTGCACATTATGGATTTTCAGAAGTGGAAGCTTATTGGCTTGCAGAGAGTGGCCTCCCCTGCCGGTG CCGGTACCCTCCATGCCCTGAGCCGCTACAGCCGCTACACGAGCATCCTGGACCTTGACAACAAAACCCT GCGCTGCCCCCTTTACAGAGGCACCCTGGTGCCCCGCCTGGCAGACCACCGCACACAGATCAAGCGGGGC AGCACCTACTACCTGCATGTCCAGAGCATGCTCACCCAGCTCTGCTCCAAGGCCTTCCTCTACACCTTCT GCCACCACCTGCACCTGCCTACCCACGACAAGGAGACAGAGGAGCTGGTAGCCAGCCGCCAGATGAGCTT CCTAAAGCTGACCCTGGGTCTGGTGAATGAGGATGTTAGGGTGGTCCAGTACCTGGCTGAGCTGCTGAAG CTGCACTACATGCAGGAATCTCCAGGGACCAGCCACCCCATGCTCAGGTTTGACTATGTCCCCAGCTTTT TGTATAAAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC212285 representing NM_144775 Red=Cloning site Green=Tags(s) MISAPDVVAFTKEEEYEEEPYNEPALPEEYSVPLFPFASQGANPWSKLSGAKFSRDFILISEFSEQVGPQ PLLTIPNDTKVFGTFDLNYFSLRIMSVDYQASFVGHPPGSAYPKLNFVEDSKVVLGDSKEGAFAYVHHLT LYDLEARGFVRPFCMAYISADQHKIMQQFQELSAEFSRASECLKTGNRKAFAGELEKKLKDLDYTRTVLH TETEIQKKANDKGFYSSQAIEKANELASVEKSIIEHQDLLKQIRSYPHRKLKGHDLCPGEMEHIQDQASQ ASTTSNPDESADTDLYTCRPAYTPKLIKAKSTKCFDKKLKTLEELCDTEYFTQTLAQLSHIEHMFRGDLC YLLTSQIDRALLKQQHITNFLFEDFVEVDDRMVEKQESIPSKPSQDRPPSSSLEECPIPKVLISVGSYKS SVESVLIKMEQELGDEEYKEVEVTELSSFDPQENLDYLDMDMKGSISSGESIEVLGTEKSTSVLSKSDSQ ASLTVPLSPQVVRSKAVSHRTISEDSIEVLSTCPSEALIPDDFKASYPSAINEEESYPDGNEGAIRFQAS ISPPELGETEEGSIENTPSQIDSSCCIGKESDGQLVLPSTPAHTHSDEDGVVSSPPQRHRQKDQGFRVDF SVENANPSSRDNSCEGFPAYELDPSHLLASRDISKTSLDNYSDTTSYVSSVASTSSDRIPSAYPAGLSSD RHKKRAGQNALKFIRQYPFAHPAIYSLLSGRTLVVLGEDEAIVRKLVTALAIFVPSYGCYAKPVKHWASS PLHIMDFQKWKLIGLQRVASPAGAGTLHALSRYSRYTSILDLDNKTLRCPLYRGTLVPRLADHRTQIKRG STYYLHVQSMLTQLCSKAFLYTFCHHLHLPTHDKETEELVASRQMSFLKLTLGLVNEDVRVVQYLAELLK LHYMQESPGTSHPMLRFDYVPSFLYKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	Chromatograms Sequencher program is needed, download here
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_144775
ORF Size	2811 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@biovalley.fr The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_144775.3
RefSeq Size	8327 bp
RefSeq ORF	2814 bp
Locus ID	140775
UniProt ID	Q8TEV9
Cytogenetics	17p11.2
MW	105 kDa
Summary	Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy (PubMed:20562859, PubMed:27193190, PubMed:27103069, PubMed:27559131, PubMed:27617292, PubMed:28195531). In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation (PubMed:20562859, PubMed:27103069, PubMed:27617292, PubMed:28195531). The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and inhibiting its protein kinase activity (PubMed:27617292, PubMed:28195531). Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates (PubMed:27559131, PubMed:28195531). In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes (PubMed:28195531).[UniProtKB/Swiss-Prot Function]