SMCR8 (NM_144775) Human Tagged ORF Clone
Référence RC212285
Conditionnement : 10ug
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| Product Data | |
| Target Symbol | SMCR8 |
|---|---|
| Synonyms | DENND8A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC212285 representing NM_144775 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAGCGCCCCTGACGTAGTGGCCTTCACCAAAGAGGAAGAGTATGAAGAAGAGCCTTACAATGAGC CGGCCCTGCCTGAGGAGTACTCGGTGCCGCTCTTCCCCTTCGCCAGTCAGGGTGCTAACCCCTGGTCAAA ACTGTCCGGGGCCAAGTTTTCGAGGGACTTCATTCTTATTTCCGAGTTCTCTGAGCAGGTGGGACCCCAA CCCTTACTGACCATCCCCAATGACACCAAAGTTTTTGGCACTTTTGATCTCAATTACTTCTCCCTGCGTA TCATGTCTGTGGATTACCAGGCTTCCTTCGTGGGCCATCCTCCTGGATCTGCCTACCCCAAGCTGAACTT CGTGGAGGACTCCAAGGTGGTGCTGGGAGATTCTAAGGAGGGCGCCTTTGCATACGTGCACCACCTTACC CTATACGACCTGGAGGCCCGTGGCTTCGTGAGGCCGTTTTGCATGGCTTATATCTCTGCAGACCAGCATA AAATCATGCAGCAGTTCCAGGAGCTTTCAGCCGAATTTTCCAGAGCTTCTGAGTGCTTGAAGACTGGCAA CAGGAAGGCATTTGCTGGGGAACTTGAAAAAAAGCTGAAAGACTTGGATTACACCAGGACAGTGCTACAC ACAGAAACGGAGATCCAGAAGAAAGCCAACGACAAAGGCTTTTACTCATCTCAGGCAATTGAGAAAGCCA ATGAACTGGCCAGTGTGGAGAAGTCCATCATTGAACATCAAGACCTGCTGAAGCAGATCCGCTCATACCC TCATCGGAAGTTGAAGGGGCATGATTTGTGTCCTGGTGAGATGGAGCACATCCAGGATCAGGCCAGCCAG GCATCCACTACCTCTAACCCTGATGAGTCTGCCGACACAGACCTTTACACCTGCAGACCAGCCTACACCC CAAAACTTATCAAAGCAAAGTCCACCAAGTGTTTTGACAAGAAGTTGAAGACCTTGGAGGAGCTCTGTGA CACTGAATATTTCACCCAGACCCTGGCTCAGCTCAGCCACATTGAACACATGTTCAGAGGAGACCTGTGT TACCTCCTGACCAGTCAGATTGATAGAGCACTTCTAAAACAACAGCATATAACAAACTTTCTCTTTGAAG ACTTTGTGGAGGTCGATGACAGGATGGTGGAGAAACAAGAAAGCATACCCTCTAAGCCCAGTCAAGACAG GCCGCCTTCCAGTTCTCTAGAAGAATGCCCAATTCCTAAAGTGTTAATTAGTGTTGGTTCTTACAAGTCC AGTGTGGAGTCTGTGTTGATCAAGATGGAGCAGGAACTGGGAGATGAGGAGTACAAGGAAGTGGAAGTGA CTGAGTTGAGCAGTTTCGACCCCCAGGAAAACTTGGACTACCTGGATATGGATATGAAAGGGAGTATCAG CAGTGGTGAAAGTATTGAAGTTTTGGGCACGGAGAAATCCACCTCCGTGCTTTCTAAATCTGACAGCCAG GCAAGCCTCACAGTACCATTGAGCCCCCAGGTGGTCCGGAGCAAAGCAGTCAGCCACAGGACCATCAGCG AGGACAGTATTGAAGTCCTCAGTACCTGCCCCTCTGAGGCCCTCATCCCTGATGACTTTAAGGCCAGCTA CCCAAGTGCCATTAATGAAGAAGAATCATATCCAGATGGCAATGAAGGAGCCATCCGCTTCCAGGCAAGC ATCAGTCCTCCAGAACTGGGTGAGACAGAGGAAGGCAGCATAGAAAACACCCCATCACAAATAGACTCCT CCTGCTGTATTGGGAAGGAGAGCGATGGTCAGTTGGTGCTGCCCTCCACTCCAGCCCACACACACTCTGA CGAGGATGGGGTGGTGAGCAGCCCCCCACAGCGCCACAGGCAGAAGGACCAGGGGTTCCGTGTAGACTTT TCAGTGGAAAATGCCAACCCTTCTTCCCGAGACAACAGTTGTGAAGGGTTTCCCGCTTATGAGCTGGACC CGAGCCACCTGCTGGCTAGCCGGGACATCAGTAAGACCAGCCTGGACAACTACTCAGACACCACCAGCTA CGTGAGCAGTGTAGCGTCCACCAGCTCAGACAGGATCCCCTCTGCTTATCCTGCTGGCCTGTCTTCCGAT AGGCATAAAAAGAGGGCTGGCCAGAACGCCTTAAAATTCATCCGCCAGTACCCCTTTGCCCACCCAGCCA TCTACTCCCTGCTCAGTGGGAGGACACTTGTGGTCCTGGGGGAAGATGAGGCCATAGTCAGGAAACTCGT GACTGCACTGGCTATCTTTGTCCCCAGCTATGGCTGCTACGCTAAGCCCGTGAAACATTGGGCCTCCTCC CCTTTGCACATTATGGATTTTCAGAAGTGGAAGCTTATTGGCTTGCAGAGAGTGGCCTCCCCTGCCGGTG CCGGTACCCTCCATGCCCTGAGCCGCTACAGCCGCTACACGAGCATCCTGGACCTTGACAACAAAACCCT GCGCTGCCCCCTTTACAGAGGCACCCTGGTGCCCCGCCTGGCAGACCACCGCACACAGATCAAGCGGGGC AGCACCTACTACCTGCATGTCCAGAGCATGCTCACCCAGCTCTGCTCCAAGGCCTTCCTCTACACCTTCT GCCACCACCTGCACCTGCCTACCCACGACAAGGAGACAGAGGAGCTGGTAGCCAGCCGCCAGATGAGCTT CCTAAAGCTGACCCTGGGTCTGGTGAATGAGGATGTTAGGGTGGTCCAGTACCTGGCTGAGCTGCTGAAG CTGCACTACATGCAGGAATCTCCAGGGACCAGCCACCCCATGCTCAGGTTTGACTATGTCCCCAGCTTTT TGTATAAAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC212285 representing NM_144775 Red=Cloning site Green=Tags(s) MISAPDVVAFTKEEEYEEEPYNEPALPEEYSVPLFPFASQGANPWSKLSGAKFSRDFILISEFSEQVGPQ PLLTIPNDTKVFGTFDLNYFSLRIMSVDYQASFVGHPPGSAYPKLNFVEDSKVVLGDSKEGAFAYVHHLT LYDLEARGFVRPFCMAYISADQHKIMQQFQELSAEFSRASECLKTGNRKAFAGELEKKLKDLDYTRTVLH TETEIQKKANDKGFYSSQAIEKANELASVEKSIIEHQDLLKQIRSYPHRKLKGHDLCPGEMEHIQDQASQ ASTTSNPDESADTDLYTCRPAYTPKLIKAKSTKCFDKKLKTLEELCDTEYFTQTLAQLSHIEHMFRGDLC YLLTSQIDRALLKQQHITNFLFEDFVEVDDRMVEKQESIPSKPSQDRPPSSSLEECPIPKVLISVGSYKS SVESVLIKMEQELGDEEYKEVEVTELSSFDPQENLDYLDMDMKGSISSGESIEVLGTEKSTSVLSKSDSQ ASLTVPLSPQVVRSKAVSHRTISEDSIEVLSTCPSEALIPDDFKASYPSAINEEESYPDGNEGAIRFQAS ISPPELGETEEGSIENTPSQIDSSCCIGKESDGQLVLPSTPAHTHSDEDGVVSSPPQRHRQKDQGFRVDF SVENANPSSRDNSCEGFPAYELDPSHLLASRDISKTSLDNYSDTTSYVSSVASTSSDRIPSAYPAGLSSD RHKKRAGQNALKFIRQYPFAHPAIYSLLSGRTLVVLGEDEAIVRKLVTALAIFVPSYGCYAKPVKHWASS PLHIMDFQKWKLIGLQRVASPAGAGTLHALSRYSRYTSILDLDNKTLRCPLYRGTLVPRLADHRTQIKRG STYYLHVQSMLTQLCSKAFLYTFCHHLHLPTHDKETEELVASRQMSFLKLTLGLVNEDVRVVQYLAELLK LHYMQESPGTSHPMLRFDYVPSFLYKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_144775 |
| ORF Size | 2811 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@biovalley.fr The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_144775.3 |
| RefSeq Size | 8327 bp |
| RefSeq ORF | 2814 bp |
| Locus ID | 140775 |
| UniProt ID | Q8TEV9 |
| Cytogenetics | 17p11.2 |
| MW | 105 kDa |
| Summary | Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy (PubMed:20562859, PubMed:27193190, PubMed:27103069, PubMed:27559131, PubMed:27617292, PubMed:28195531). In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation (PubMed:20562859, PubMed:27103069, PubMed:27617292, PubMed:28195531). The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and inhibiting its protein kinase activity (PubMed:27617292, PubMed:28195531). Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates (PubMed:27559131, PubMed:28195531). In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes (PubMed:28195531).[UniProtKB/Swiss-Prot Function] |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC212285L1 | Lenti-ORF clone of SMCR8 (Myc-DDK-tagged)-Human Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8) | 10 ug | | |
| RC212285L2 | Lenti-ORF clone of SMCR8 (mGFP-tagged)-Human Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8) | 10 ug | | |
| RC212285L3 | Lenti-ORF clone of SMCR8 (Myc-DDK-tagged)-Human Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8) | 10 ug | | |
| RC212285L4 | Lenti-ORF clone of SMCR8 (mGFP-tagged)-Human Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8) | 10 ug | | |
| RG212285 | SMCR8 (tGFP-tagged) - Human Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8) | 10 ug | | |
| SC318400 | SMCR8 (untagged)-Human Smith-Magenis syndrome chromosome region, candidate 8 (SMCR8) | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.