Scnn1g polyclonal antibody (ATTO 390)

Product Description

Rabbit polyclonal antibody raised against recombinant Rat Scnn1g.

Immunogen

Recombinant protein corresponding to Scnn1g C-terminus (amino acids 629-650).

Host

Rabbit

Reactivity

Mouse, Rat

Specificity

1 ug/mL of PAB28803 was sufficient for detection of Scnn1g, ~83kDa, in 20ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Form

Liquid

Conjugation

ATTO 390

Purification

Affinity purification

Recommend Usage

Immunofluorescence
Immunohistochemistry
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Storage Buffer

PBS, 50% glycerol and 0.09% sodium azide

Storage Instruction

Store at 4°C.
Aliquot to avoid repeated freezing and thawing.

Note

Application Data with Unconjugated Antibody

Western Blot (Tissue lysate)

Western blot analysis of Scnn1g in mCCD (Mouse Cortical Collecting Duct) and FRT cells (mouse kidney) with Scnn1g polyclonal antibody (Cat # PAB28802) at 1:1000 dilution.
Lane 1: mCCD cell lysate
Lane 2: FRT cells expressing tagged Scnn1g
Lane 3: FRT cell lysate
Lane 4: FRT cells expressing tagged Scnn1g

Immunofluorescence

Immunofluorescence staining of Scnn1g in rat kidney with Scnn1g polyclonal antibody (Cat # PAB28802).