S100B ELISA Kit (Human)
Référence OKEH02888
Conditionnement : 96Wells
Marque : Aviva Systems Biology
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
S100B ELISA Kit (Human) : 96 Wells (OKEH02888)
| Datasheets/Manuals | Printable datasheet for S100B ELISA Kit (Human) : 96 Wells (OKEH02888) |
|---|---|
| COA Datasheet | Printable COA datasheet for OKEH02888 |
| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology S100B ELISA Kit (Human) (OKEH02888) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100B captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 15.6-1000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | <= 6.4% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 3.9% (n = 20) Mean Inter-assay CV%: < 6.7% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 7.8 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Protein S100-B | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | S100B |
|---|---|
| Gene Full Name | S100 calcium binding protein B |
| Alias Symbols | NEF, protein S100-B, S-100 calcium-binding protein, beta chain, S-100 protein beta chain, S-100 protein subunit beta, S100, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S100-B, S100beta. |
| NCBI Gene Id | 6285 |
| Protein Name | Protein S100-B |
| Description of Target | Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. |
| Uniprot ID | P04271 |
| Protein Accession # | NP_006263.1 |
| Nucleotide Accession # | NM_006272.2 |
| Protein Size (# AA) | 92 |
| Molecular Weight | 10 kDa |
