Retrovirus Precipitation Reagent

Référence VC200

Conditionnement : 100mL

Marque : Alstem

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Retrovirus Precipitation Reagent

Highlights:
  • Easy-to-use: simply mix
  • No ultracentrifugation required
  • Easily scale up for large volumes
  • Up to 100 fold concentration increase
  • Cost effective spin protocol for efficient viral concentration
  • Non-toxic: safe for all cell lines, including ES cells
Description:

Lentivirus Precipitation Solution is a mixture of polymers optimized for the precipitation of lentiviral particles. It provides a simple, fast and highly efficient method for concentrating lentiviral particles. The protocol involve mixing your lentiviral supernatant with the Lentivirus Precipitation Solution, incubate for a short period, and spin the mixture in a standard centrifuge. You'll increase your lentivirus titer by up to 100 fold as quick as in 4 hrs, and obtain excellent recoveries without ultracentrifugation. The Lentivirus Precipitation Solution is a 5x solution.

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Specifications:
Product Name Retrovirus Precipitation Reagent
Shipping Condition

Room Temperature - 2 Day Shipping

Storage and Stability

Store at 4 °C. This product is stable for 6 months when stored as directed

Quality Control

Each lot of Retrovirus Precipitation Solution is tested for sterility and successful precipitation of Lentiviral particles.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

  1. K. Sauls et al., Initiating Events in Direct Cardiomyocyte Reprogramming. Cell Rep 22, (2018).

  2. H. Ma et al., Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarction. Methods Mol Biol 1521, (2017).
  3. L. Wang et al., Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency and quality of induced cardiac myocyte reprogramming. Circ Res 116, (2015).

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