Recombinant Human BDNF
Référence 100-01-10UG
Conditionnement : 10ug
Marque : FUJIFILM
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Recombinant Human BDNF
Brain-derived neurotrophic factor (BDNF) is a nerve growth factor that binds two receptors, the low-affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase B (TrkB), to support neuron growth and survival. BDNF expression in the hippocampus is essential for long-term memory storage and learning. Human, mouse, rat, and pig BDNF are cross-reactive.
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Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | None |
| Accession Number | P23560 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Noncovalent Homodimer, 13.6/27.3 kDa (120/240 aa) |
| AA Sequence | MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | Proliferation of a neuroblastoma cell line stably expressing TrkB (BR6) | ≤ 40 ng/mL; 2.5 x 104 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.