Rat S100A8 (S100 Calcium Binding Protein A8) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	S100a8
Gene ID:	116547
Uniprot ID:	S10A8_RAT
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Tissue Specificity
Post Translational Modifications
Function	S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect.regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, ANXA5, EZR, MSN and VIM by recognizing a IL-x-C-x-x-DE motif.S100A8 seems to contribute to S-nitrosylation site selectivity.
Protein Name	Protein S100-A8 Calgranulin-A Migration Inhibitory Factor-Related Protein 8 Mrp-8 P8 S100 Calcium-Binding Protein A8
Database Links	Reactome: R-RNO-5668599 Reactome: -RNO-6798695 Reactome: -RNO-6799990
Cellular Localisation	Secreted Cytoplasm Cytoskeleton Cell Membrane Peripheral Membrane Protein Predominantly Localized In The Cytoplasm Upon Elevation Of The Intracellular Calcium Level Translocated From The Cytoplasm To The Cytoskeleton And The Cell Membrane Upon Neutrophil Activation Or Endothelial Adhesion Of Monocytes Is Secreted Via A Microtubule-Mediated Alternative Pathway
Alternative ELISA Names	Protein S100-A8 ELISA kit Calgranulin-A ELISA kit Migration Inhibitory Factor-Related Protein 8 ELISA kit Mrp-8 ELISA kit P8 ELISA kit S100 Calcium-Binding Protein A8 ELISA kit S100a8 ELISA kit Mrp8 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance