Rat Anti-Human IL-3-LE/AF

Référence 10115-14

Conditionnement : 0.5mg

Marque : Southern Biotech

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Clone BVD8-3G11
Isotype Rat IgG1κ
Isotype Control Rat IgG1-LE/AF (KLH/G1-2-2)
Specificity Human IL-3
Alternative Names Interleukin-3, burst promoting activity, BP/BPA, eosinophil colony stimulating factor, Eo-CSF, hematopoietic cell growth factor, HCGF, mast cell growth factor, MGF, MCGF, multi-colony stimulating factor, multi-CSF, P cell stimulating activity, PCSA
Immunogen Yeast-expressed human IL-3
Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Handle under aseptic conditions
Applications ELISA-Capture – Quality tested 1-3
Western Blot – Reported in literature 1
Immunoprecipitation – Reported in literature 1
Neutralization – Reported in literature 1,2,5

Note – May be paired with the biotinylated clone BVD3-1F9 (SB Cat. No. 10116-08) in a sandwich ELISA

RRID Number AB_2794146
Gene ID 3562 (Human)
Gene ID Symbol IL3 (Human)
Gene ID Aliases IL-3; MCGF; MULTI-CSF
UniProt ID P08700 (Human
UniProt Name IL3_HUMAN (Human)
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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  1. 1. Kaushansky K, Shoemaker SG, Broudy VC, Lin NL, Matous JV, Alderman EM, et al. Structure-function relationships of interleukin-3. An analysis based on the function and binding characteristics of a series of interspecies chimera of gibbon and murine interleukin-3. J Clin Invest. 1992;90:1879-88. (Immunogen, ELISA-Capture, WB, IP, Neut)
  2. 2. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
  3. 3. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Capture)
  4. 4. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-ed anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
  5. 5. Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (Neut)