Revolutionary Technology In Downstream Processing

The Thomson Optimum Growth® System of products is expanding into downstream processing with a revolutionary new technology that allows high speed clarification of cellular material. Thomson developed the Rapid Clear® Cap to address the needs of scientists to quickly and efficiently clarify cell culture media directly from the Thomson 5L Optimum Growth® shaker flask without the need for centrifugation.

• Depth filtration with a 0.2μm final pore size eliminates multiple filtration steps and in most cases centrifugation

• Significant time savings versus traditional spin down technique

• Cell culture clarification of low or high density cultures of CHO stable, CHO transient, HEK293, hybridoma, and other mammalian cell lines

• Eliminates transfer steps: The Rapid Clear® Cap screws directly onto the Optimum Growth® Flask

• Secondary cap attaches to a new Optimum Growth® Flask or to a storage container with a Luer lock

• Save time, clarify 3L of cell culture in less than 30 minutes - with no centrifugation required!

• Reduce consumables used by up to 90%

• Walk away convenience and safety - minimize endotoxin exposure

Simply switch out the vented Optimum Growth® Cap for the Rapid Clear®. The only equipment required is a peristaltic pump. In initial testing the units can filter up to 2.5L-3L of high density culture, 6x10^6 -20x10^6 @ >70% viability, in approximately 18 minutes. This technique will transform the time consuming and laborious task of harvesting cells to a rapid walk away procedure.

HPLC was utilized to quantitate intact protein for each of the purified solutions. The Rapid Clear® System yielded a slightly higher quantity of intact IgG, than the GE capsule columns.

Both GE and Rapid Clear® clarified IgG were run on an SDS Page gel for comparison: Lanes 1 & 4 are molecular weight standard ladder; Lanes 2&3 are non-reduced; lanes 5&6 are reduced.

• 1 ug of post proA protein per lane
• Both Reduced and Non-reduced samples
• 4-12% Bis Tris Gel from Life Tech
• Run in MES buffer @ 200 V for 30 minutes
• Stained with Safe Stain
• Ladder is Precision Plus from Biorad

A= GE filtered material
B= Rapid Clear®filtered material

Reduce Operating Costs by Increasing Efficiency & Minimizing the Number of Consumables Used

When producing biologics, cell yield and viability, and effective clarification are critical. Thomson's patented Optimum Growth® Flask design facilitates good mixing and high gas exchange rates to produce high density yields of viable cells. Thomson has used its expertise in filtration to develop the Rapid Clear® Cap 3000 to speed up the clarification process. This innovation reduces operating costs by increasing efficiency and minimizing the number of consumables used.

Quick & Easy to Use

Using the Rapid Clear® Cap 3000 is quick and easy. Once the mammalian or insect cell culture is ready for clarification, simply remove the vent cap and replace it with the Rapid Clear® Cap 3000. The chart provides the approximate time^*** to clarify cell cultures based on cell type, viability and volume to be filtered. The higher the viability the faster the filtration.