E-OSEL-B0001-96T | QuicKey Pro Bovine E2 (Estradiol) ELISA Kit Biovalley

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	5.87 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reacitivity	Bovine
Specificity	This kit recognizes Bovine E2 in samples.No significant cross-reactivity or interference between Bovine E2 and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine E2 are added to the micro ELISA plate wells. Bovine E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Bovine E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Estradiol, western drug name.Commonly used dosage forms are gels, patches, compound tablets, etc.For estrogen drugs.It is used for the following symptoms caused by estrogen deficiency caused by various reasons: hot flashes, sweating, sleep disorders, dizziness, genital atrophy, atrophic vaginitis, vaginal dryness, etc.

Research Area

Cell Biology , Metabolism

QuicKey Pro Bovine E2 (Estradiol) ELISA Kit

Référence E-OSEL-B0001-96T

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