Proline (Pro) Colorimetric Assay Kit

Référence E-BC-K177-S-100

Conditionnement : 100Assays

Marque : Elabscience

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Detection Principle Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm.
Synonyms Pro
Sample Type Plant tissue,Honey
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (520 nm)
Research Area Amino Acids And Proteins ,  Plant Physiology Series
Other Reagents Required Acetic acid, Methylbenzene, Absolute ethyl alcohol
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.17 μg/mL
Detection Range 0.17-35 μg/mL
Precision inter-assay CV: 5.4% | intra-assay CV: 4.5%
Assay Time 1 h 20 min
Cat.No. Product Name Sizes
E-BC-K118-S Glutamic Acid Colorimetric Assay Kit 100Assays , 50Assays
E-BC-K130-S Pyruvic Acid Colorimetric Assay Kit 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Green pepper tissue homogenization 1
Carrot tissue homogenization 1
Epipremnum aureum tissue homogenization 1
Lettuce leaf tissue homogenization 1
Honey 1
Cabbages tissue homogenization 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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