Polyethylenimine Linear(PEI), MW40000
Référence K1029-8
Conditionnement : 8mL(2.5mg/mL)
Marque : APExBIO Technology
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Polyethylenimine Linear(PEI), MW40000
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Linearized polyethyleneimine PEI (molecular weight 40,000) is a highly charged cationic polymer that readily binds to DNA or other negatively charged biomacromolecules, making it a common and effective cell transfection reagent. In principle, PEI condenses DNA plasmid into positively charged complexes. The complexes can adhere to negatively charged cell surface residues, and then enters the cell through endocytosis.
As a transient transfection reagent, PEI has the advantages of high efficiency, low cost and relative stability, etc., which has been validated for a wide range of common cell lines including HEK-293, HEK293T, CHO-K1, HepG2 and Hela cell transfection. In HEK293 and CHO cell expression systems, PEI provides excellent transfection results at different sizes (from 96-well plates to 100 L bioreactors).
- 1. Yu Huang, Lu Li, et al. "WHIM Syndrome: Report of a Novel Familial CXCR4V340fs Gain-of-function Mutation with a Milder Phenotype." Research Square. 13 Nov, 2023.
Protocol
| Components | 4 mL (2.5 mg/mL) | 8 mL (2.5 mg/mL) |
| PEI-2.5 mg/mL | 4 mL | 8 mL |
| 2X Transfection Buffer | 3 mL | 6 mL |
For long-term storage, store at -20°C. For frequent use, it can be placed at 4°C to avoid repeated freeze-thaw cycles. | ||