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pLenti-P2A-Puro Lentiviral Gene Expression Vector

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Référence PS100109

Conditionnement : 10ug

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pLenti-P2A-Puro Lentiviral Gene Expression Vector

pLenti-P2A-Puro Lentiviral Gene Expression Vector

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SKU

PS100109

pLenti-P2A-Puro, tag free Lenti vector with P2A-Puro, 10 ug

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Datasheet SDS Vector Sequence

In Stock*

Specifications

Specifications
Product Data
E. coli Selection	Chloramphenicol (34 ug/mL)
Mammalian Cell Selection	Puromycin
Features	This Lenti vector is a third generation lentiviral vector. All OriGene's TrueORF clones can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF (no stop codon for P2A-Puro) cloned in this vector will be expressed as native protein, no tag. P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli. selection: Chloramphenicol (34 ug/ml) V2_F Primer can be used to sequence the target sequence after cloning. P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites

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Documents

Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors
SDS for PS100109

Resources

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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pLenti-P2A-Puro Lentiviral Gene Expression Vector

Référence PS100109

Contactez votre distributeur local :

pLenti-P2A-Puro Lentiviral Gene Expression Vector

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