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> pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

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Conditionnement : 10ug

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pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

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PS100093
pLenti-C-mGFP-P2A-Puro, Lenti vector with C-terminal mGFP tag and P2A-Puro, 10 ug
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Datasheet SDS Vector Sequence
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Specifications
Product Data
C-Tag mGFP
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Expression Promoter CMV
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal monomeric GFP tag.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol (34ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • mGFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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