PIPES [5625-37-6]
Référence HY-D0875-1ml
Conditionnement : 10mM/1mL
Marque : MedChemExpress
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PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry.
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PIPES Chemical Structure
CAS No. : 5625-37-6
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| Description |
PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry[1]. |
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| In Vitro |
To prepare the pH PIPES buffer, 173 g of 1,4-piperazinediethanesulfonic acid are dissolved into 1 L of deionized water. The pH of the PIPES buffer is adjusted to 6.8 by adding pellets of sodium hydroxide. PIPES can be used to PIPES buffer, it can prevent the glutaraldehyde fixation induced lipid loss and artifacts[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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| Masse moléculaire |
302.37 |
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| Formule |
C8H18N2O6S2 |
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| CAS No. |
5625-37-6 |
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| Appearance |
Solid |
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| Color |
White to off-white |
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| SMILES |
O=S(CCN1CCN(CCS(=O)(O)=O)CC1)(O)=O |
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| Livraison | Room temperature in continental US; may vary elsewhere. |
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| Stockage |
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| Solvant et solubilité |
In Vitro:
H2O : 5 mg/mL (16.54 mM; ultrasonic and adjust pH to 7 with NaOH) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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| Pureté et documentation |
Purity: ≥99.0% |
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| Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|
| H2O | 1 mM | 3.3072 mL | 16.5360 mL | 33.0721 mL | 82.6802 mL |
| 5 mM | 0.6614 mL | 3.3072 mL | 6.6144 mL | 16.5360 mL | |
| 10 mM | 0.3307 mL | 1.6536 mL | 3.3072 mL | 8.2680 mL | |
| 15 mM | 0.2205 mL | 1.1024 mL | 2.2048 mL | 5.5120 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
PIPES Related Classifications
- Inflammation/Immunology Cancer
- Cancer Metabolism and Metastasis
- Others
- Biochemical Assay Reagents
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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
PIPES5625-37-61,4-Piperazinediethanesulfonic acidBiochemical Assay Reagentsbuffer PIPEScell culturesample prepareglutaraldehyde fixativeInhibitorinhibitorinhibit