Phosphoenolpyruvate Carboxylase (PEPC) Activity Assay Kit
Référence E-BC-K613-M-48T
Conditionnement : 48T
Marque : Elabscience
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
| Detection Principle | Phosphoenolpyruvate carboxylase (PEPC) catalyzes phosphoenolpyruvate (PEP) to produce oxaloacetic acid, which further reacts with NADH. NADH has maximum absorption at 340 nm. The activity of PEPC can be calculated by measuring the change of absorbance value at 340 nm. |
| Synonyms | PEPC |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (320-360 nm, optimum wavelength: 340 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.72 U/L |
| Detection Range | 0.72-25.61 U/L |
| Precision | inter-assay CV: 8.5-10.6% | intra-assay CV: 1.5-4.3% |
| Sample Volume | 40 μL(tissue homogenate) |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Shanghai green tissue homogenate | 1 |
| 10% Sweet potato leaves tissue homogenate | 1 |
| 10% Crowndaisy chrysanthemum tissue homogenate | 1 |
| 10% Napa cabbage tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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