TP323190 | PEAMT (PEMT) (NM_007169) Human Recombinant Protein Biovalley

PEAMT (PEMT) (NM_007169) Human Recombinant Protein

SKU

TP323190

Recombinant protein of human phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 2, 20 µg

MVPro

Full-length human proteins expressed in HEK293T cells

Specifications
Product Data
Species	Human
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence >RC223190 representing NM_007169 Red=Cloning site Green=Tags(s) MTRLLGYVDPLDPSFVAAVITITFNPLYWNVVARWEHKTRKLSRAFGSPYLACYSLSVTILLLNFLRSHC FTQAMLSQPRMESLDTPAAYSLGLALLGLGVVLVLSSFFALGFAGTFLGDYFGILKEARVTVFPFNILDN PMYWGSTANYLGWAIMHASPTGLLLTVLVALTYIVALLYEEPFTAEIYRQKASGSHKRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Predicted MW	22 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation	Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping	Dry Ice
Reference Data
RefSeq	NP_009100
Locus ID	10400
UniProt ID	Q9UBM1
Cytogenetics	17p11.2
RefSeq Size	1008
RefSeq ORF	597
Synonyms	PEAMT; PEMPT; PEMT2; PLMT; PNMT
Summary	Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]
Protein Families	Transmembrane
Protein Pathways	Glycerophospholipid metabolism, Metabolic pathways

FAQs
Protein FAQs
SDS
SDS for TP323190

Recombinant Protein Resources
Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

SKU	Description	Size
PH323190	PEMT MS Standard C13 and N15-labeled recombinant protein (NP_009100)	10 ug
LC402097	PEMT HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LC407769	PEMT HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LC407770	PEMT HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LY402097	Transient overexpression lysate of phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 2	100 ug
LY407769	Transient overexpression lysate of phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 1	100 ug
LY407770	Transient overexpression lysate of phosphatidylethanolamine N-methyltransferase (PEMT), nuclear gene encoding mitochondrial protein, transcript variant 3	100 ug

PEAMT (PEMT) (NM_007169) Human Recombinant Protein

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PEAMT (PEMT) (NM_007169) Human Recombinant Protein

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