PCGF1 (Polycomb group RING Finger Protein 1, Nervous system Polycomb-1, NSPc1, RING Finger Protein 68, PCGF1, NSPC1, RNF68)

Polycomb group (PCG) proteins form multiprotein complexes that regulate expression patterns of developmental and cell proliferation genes. Several PCG proteins contain ring finger domains and have been identified as a subclass of RING finger proteins. The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain the RING-type zinc finger conserved domain are generally involved in the ubiquitination pathway of protein degradation. PCGF1 (polycomb group ring finger 1), also known as NSPC1, RNF68 or RNF3A-2, is a 259 amino acid nuclear protein that is ubiquitously expressed. PCGF1 is a component of the PCG multiprotein BCoR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as Bcl-6 and the cyclin-dependent kinase inhibitor, p21. PCGF1 promotes cell cycle progression and enhances cell proliferation.||Applications: |Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Western Blot: 1:500-1:2000|Immunohistochemistry: 1:50-1:200|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.