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Mouse Monoclonal Anti-Involucrin (Squamous Cell Terminal Differentiation Marker) [Clone SY5]
Référence NB-36-00394-P1
Conditionnement : 100ug
Marque : Neo Biotech
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Involucrin (Squamous Cell Terminal Differentiation Marker) Antibody [SY5]
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Formalin-fixed, paraffin-embedded human Tonsil stained with Involucrin Mouse Monoclonal Antibody (SY5).
SDS-PAGE Analysis of Purified Involucrin Mouse Monoclonal Antibody (SY5). Confirmation of Purity and Integrity of Antibody.
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Dog, Gorilla, Human, Owl monkey, Pig
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm
Gene Name
IVL
Positive Control
MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Immunogen
Purified involucrin from human keratinocytes
Alternate Names
Involucrin, IVL
Functions
- Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Key References
- Hudson DL, et. al. Hybridoma, 1992, 11:367-79.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2873896/
- https://pubmed.ncbi.nlm.nih.gov/16710414/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/9115270/
- https://pubmed.ncbi.nlm.nih.gov/1601889/
- https://pubmed.ncbi.nlm.nih.gov/2461365/
- https://pubmed.ncbi.nlm.nih.gov/9651377/
- https://pubmed.ncbi.nlm.nih.gov/15147942/
- https://pubmed.ncbi.nlm.nih.gov/21269460/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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