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Mouse Monoclonal Anti-IDH1 (Isocitrate Dehydrogenase) [Clone IDH1/1152]
Référence NB-36-00310-P1
Conditionnement : 100ug
Marque : Neo Biotech
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IDH1 (Isocitrate Dehydrogenase) Antibody [IDH1/1152]
Catalog Number Formulation Size Price
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with IDH1 Mouse Monoclonal Antibody (IDH1/1152).
Immunofluorescence Analysis of PFA-fixed HeLa cells labeling IDH1. IDH1 Mouse Monoclonal Antibody (IDH1/1152) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.
IDH1 Antibody in Western Blot (WB)
Western Blot Analysis of A-549 lysate using IDH1 Mouse Monoclonal Antibody (IDH1/1152).
IDH1 Antibody in Western Blot (WB)
Western Blot Analysis of Human Kidney tissue lysate using IDH1 Mouse Monoclonal Antibody (IDH1/1152).
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol
Summary
It recognizes a 45kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming 伪-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of 伪-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Infectious Disease, Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Cytosol, Peroxisome
Gene Name
IDH1
Positive Control
Human breast, Kidney colon or prostate carcinoma. HeLa, HepG2, HT29 or MCF7 cells, A-549
Immunogen
Recombinant fragment of human IDH1 protein (around aa 281-414) (exact sequence is proprietary)
Alternate Names
Isocitrate dehydrogenase [NADP] cytoplasmic, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, Cytosolic NADP-isocitrate dehydrogenase; Epididymis luminal protein 216; Epididymis secretory protein Li 26; HEL-216; HEL-S-26; ICDH; IDCD; IDH1; IDP; IDPC; Isocitrate dehydrogenase 1 (NADP+) soluble; NADP dependent isocitrate dehydrogenase peroxisomal; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; PICD
Functions
- Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
Key References
- Geisbrecht, B.V. and Gould, S.J. 1999. J. Biol. Chem. 274: 30527-30533.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/9866202/
- https://pubmed.ncbi.nlm.nih.gov/10521434/
- https://pubmed.ncbi.nlm.nih.gov/11230166/
- https://pubmed.ncbi.nlm.nih.gov/17974005/
- https://pubmed.ncbi.nlm.nih.gov/15815621/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/19608861/
- https://pubmed.ncbi.nlm.nih.gov/21269460/
- https://pubmed.ncbi.nlm.nih.gov/22814378/
- https://pubmed.ncbi.nlm.nih.gov/24275569/
- https://pubmed.ncbi.nlm.nih.gov/25944712/
- https://pubmed.ncbi.nlm.nih.gov/15173171/
- https://pubmed.ncbi.nlm.nih.gov/19935646/
- https://pubmed.ncbi.nlm.nih.gov/16959974/
- https://pubmed.ncbi.nlm.nih.gov/18772396/
- https://pubmed.ncbi.nlm.nih.gov/19117336/
- https://pubmed.ncbi.nlm.nih.gov/26161668/
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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