Interferon Beta (IFNb) Recombinant, Human

Recombinant protein corresponding to aa22-187 with an N-Terminal His-Tag from human IFNb, expressed in E. coli. Swiss/UniProt Accession: P01574.||Amino Acid Sequence:|MSYNLLGFL QRSSNFQCQK LLWQLNGRLE YCLKDRMNFD IPEEIKQLQQ FQKEDAALTI YEMLQNIFAI FRQDSSSTGW NETIVENLLA NVYHQINHLK TVLEEKLEKE DFTRGKLMSS LHLKRYYGRI LHYLKAKEYS HCAWTIVRVE ILRNFYFINR LTGYLRN||Predicted Isoelectric Point:|8.9||Predicted Molecular Weight:|23.7kD||Accurate Molecular Mass:|25kD as determined by SDS-PAGE reducing conditions||Subcellular Location:|Secreted||Applications:|Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||Note:|Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.