IL12B Mouse Monoclonal Antibody [Clone ID: CC301]
Référence AM03219LE-N
Conditionnement : 500ug
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| Product Data | |
| Clone Name | CC301 |
|---|---|
| Application | ELISA, Elispot, FC, FN, WB |
| Application | ELISA: 8 µg/ml. This antibody may be used as a capture antibody in a Sandwich ELISA in combination with Biotinylated clone CC326 (Cat.-No AM05876BT-N) as detection reagent (See Bannerman, D.D.et al.) ELISpot. Western Blot: 1/50-1/100. Flow Cytometry: Use 10 µl of 1/10 diluted antibody to label 106 cells in 100 µl. Membrane permeabilisation is required. Functional Assays: 1/100. |
| Reactivity | Bovine, Human, Sheep |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Recombinant Bovine IL-12. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
| Specificity | This antibody recognises the p40 subunit of Bovine Interleukin-12, and binds to both the free subunit and the intact heterodimer. Negative Species: Mouse. |
| Buffer | PBS State: Low Endotoxin State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: None Preservative: None |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Database Link | |
| Background | Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10; homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35. |
| Synonyms | Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2 |
| Reference Data | |
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