IL-4, Anti-Rat
Référence NB-50-1201-100
Conditionnement : 100µg
Marque : Neo Biotech
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Western Blot: To detect Rat IL-4 by Western Blot analysis IL-4 Polyclonal Antibody (Product # 500-P94-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot: To detect Rat IL-4 by Western Blot analysis IL-4 Polyclonal Antibody (Product # 500-P94-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA: To detect Rat IL-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-4 Polyclonal Antibody (Product # 500-P94-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-4 Polyclonal Antibody, Biotin (Product # 500-P94BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-4.
Published figure using IL-4 polyclonal antibody (Product # 500-P94-100UG) in Neutralization
Published figure using IL-4 polyclonal antibody (Product # 500-P94-100UG) in Neutralization
Product Details
500-P94-100UG
Species Reactivity
Published species
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Immunogen
Conjugate
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Product Specific Information
AA Sequence of recombinant protein: MHGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL TTLKDFLESL KSILRGKYLQ SCTSMS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-4. Anti-Rat IL-4-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-4 matrix.
Sandwich ELISA: To detect Rat IL-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-4 (500-P94Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-4.
Western Blot: To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.