IgM (AP)
Référence I1905-01U-1mg
Conditionnement : 1mg
Marque : US Biological
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I1905-01U IgM (AP)
Clone Type
PolyclonalHost
goatSource
ratIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
Hu RtShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeApplications: |Suitable for immunoblot, ELISA, immunohistochemistry and other phosphatase-antibody based enzymatic assays |Other applications not tested.||Recommended Dilutions: |ELISA: 1:500-1:2000 (Coat with1ug of Rat IgM).|Optimal dilutions should be determined by the researcher.||Label: |Alkaline Phosphatase (Calf Intestine) ||Molecular Weight: |140kD||Storage and Stability:|Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: rat|Concentration: ~1mg/ml (UV280)|Form: Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Rat IgM heavy chain.|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgM and Rat Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other Rat or human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat IgM heavy chain.
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgM and Rat Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other Rat or human heavy or light chain isotypes.