IgE monoclonal antibody, clone BE5 (FITC)

Catalog # MAB4491

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Price

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Quantity

Size:100 ug
Price: USD $ 329.00
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Images
Flow Cytometry
Application

Flow Cytometry

Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody.
Combination of IgE monoclonal antibody, clone BE5 (FITC) (Cat # MAB4491) and IgE monoclonal antibody, clone BE5 (PE) (Cat # MAB4492)-Dyomics 647 was used (analysis in basophil wIDO1w).
Fig. A - staining of non-stimulated (control) sample.
Fig. B - staining of IgE stimulated sample.

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against IgE.

    Immunogen

    Human IgE.

    Host

    Mouse

    Reactivity

    Human

    Specificity

    This antibody reacts with human IgE; it recognizes an epitope different from the ones recognized by MAB6451 and MAB6452 antibodies to IgE.

    Form

    Liquid

    Conjugation

    FITC

    Concentration

    0.1 mg/mL

    Isotype

    IgG

    Recommend Usage

    Flow Cytometry (5 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.2% BSA, 0.09% sodium azide)

    Storage Instruction

    Store in the dark at 4°C. Do not freeze.
    Avoid prolonged exposure to light.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Enzyme-linked Immunoabsorbent Assay

    Flow Cytometry

    Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody.
    Combination of IgE monoclonal antibody, clone BE5 (FITC) (Cat # MAB4491) and IgE monoclonal antibody, clone BE5 (PE) (Cat # MAB4492)-Dyomics 647 was used (analysis in basophil wIDO1w).
    Fig. A - staining of non-stimulated (control) sample.
    Fig. B - staining of IgE stimulated sample.
  • Publication Reference