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Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Hydrogen Peroxide (H2O2) Colorimetric Assay Kit

Imprimer

Référence E-BC-K102-M-96T

Conditionnement : 96T

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Marque : Elabscience

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Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Detection Principle	Hydrogen peroxide can react with ammonium molybdate to form a yellow complex which has a maximum absorption peak at 405 nm. H₂O₂ content can be calculated by measuring the absorbance value at 405 nm.
Sample Type	Serum,Plasma,Urine,Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (402-407 nm, optimum wavelength: 405 nm)
Research Area	Oxidative Stress , Plant Physiology Series , Ferroptosis
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.41 mmol/L
Detection Range	0.41-125 mmol/L
Precision	inter-assay CV: 3.6% \| intra-assay CV: 3.2%
Sample Volume	15 μL
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-F001	Hydrogen Peroxide (H2O2) Fluorometric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Rat serum	1
Mouse serum	1
Mouse plasma	1
Porcine serum	1
Human urine	1
Cell homogenate	1
10% Plant tissue homogenate	1
10% Rat heart tissue homogenate	1
10% Rat brain tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat liver tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.