Human IL-6 ELISA Kit (96-well)
Référence EA100030
Conditionnement : 1x96wells
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| Product Data | |
| ACCN | NM_000600 |
|---|---|
| Assay Length | 3 hours |
| Assay Platform | 96-well plate |
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Type | Sandwich |
| Background | Interleukin 6(IL-6) is a multifunctional protein produced by lymphoid and non-lymphoid cells and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. For reviews on IL-6, see references 1-5. The human IL-6 cDNA sequence predicts a protein of 212 amino acid (aa) residues in length with two potential N-glycosylation sites. The hydrophobic N-terminal 28 aa residue signal peptide is cleaved to produce a mature protein of 184 amino acids with four cysteine residues and a predicted molecular mass of 21 kDa . The mouse IL-6 cDNA sequence shows a homology of 42% at the aa level when compared with the human sequence . On the basis of sequence similarity and gene structural motif similarity, IL-6 can be grouped in a family of cytokines that also includes OSM, G-CSF, LIF, and CNTF. All of these cytokines are predicted to have a four helix bundle structure similar to that found for growth hormone, suggesting that they all evolved from a common ancestral gene . The effects of IL-6 on different cells are numerous and varied. The effect on B cells is stimulation of differentiation and antibody secretion . IL-6 also affects T cells, acting as a co-stimulant with sub-optimal concentrations of PHA or Con A to stimulate IL-2 production and IL-2 receptor expression. IL-6 exhibits growth factor activity for mature thymic or peripheral T-cells and reportedly enhances the differentiation of cytotoxic T-cells in the presence of IL-2 or IFN-. . IL-6 stimulates production of acute phase proteins by hepatocytes and has colony-stimulating activity on hematopoietic stem cells. IL-6 has growth factor activities and will stimulate the growth of myeloma/hybridoma/ plasmacytoma cells, EBV-transformed B cells, keratinocytes and mesangial cells . |
| Components | Aluminium pouches with a Microwell Plate coated with antibody to human IL-6 (8 x 6) 2 vials human IL-6 Standard lyophilized, 1000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IL-6 antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 4 pieces Adhesive Films package insert |
| Curve Range | 3.9-250pg/ml |
| figure_legend | Representative standard curve for IL-6 ELISA. IL-6 was diluted in serial two-fold steps in Sample Diluent. |
| Format | 48-well strip plate |
| Gene Symbol | IL6 |
| Interference | No significant interference observed with available related molecules. |
| Locus ID | 3569 |
| Reactivity | Human |
| Reactivity | Human |
| Reactivity | Human |
| Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
| Sample Volume | 20 uL |
| Sensitivity | 2pg/mL |
| Signal | Colorimetric |
| Species | Human |
| Specificity | Natural and recombinant Human IL-6 Ligand |
| Standard Curve | /assets/images/elisa/kits/128/che0009-096-1-e.jpg |
| Target Symbol | IL6 |
| Description | Human IL-6 ELISA Kit (96-well) |