Human IgE Mouse Monoclonal Antibody [Clone ID: NI 307]

Référence AM20251FC-N

Conditionnement : 200ug

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Human IgE Mouse Monoclonal Antibody [Clone ID: NI 307]

Specifications
Product Data
Clone Name NI 307
Application ELISA, IF, IP, WB
Application To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
Reactivity Human
Antibody Host Mouse
Clonality Monoclonal
Immunogen Highly purified polyclonal IgE isolated from Human serum.
Specificity The reactivity of the antiserum is restricted to an isotype specific determinant of the IgE molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Buffer PBS, pH 7.2 without preservatives or foreign proteins
Label: FITC
State: Lyophilized purified Ig fraction
Label: Fluorescein Isothiocyanate
Absorption emission: 492 nm / 515 nm
Molar radio: Fluorochrome/IgG ~3.8
Reconstitution Method Restore by adding 0.5 ml sterile distilled water
Concentration 0.4 mg/ml
Conjugation FITC
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
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